Atomistry » Caesium » PDB 2ov4-4ht3 » 2tpl
Atomistry »
  Caesium »
    PDB 2ov4-4ht3 »
      2tpl »

Caesium in PDB 2tpl: Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion

Enzymatic activity of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion

All present enzymatic activity of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion:
4.1.99.2;

Protein crystallography data

The structure of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion, PDB code: 2tpl was solved by A.A.Antson, T.V.Demidkina, K.S.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 15.00 / 2.50
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 135.070, 143.910, 59.800, 90.00, 90.00, 90.00
R / Rfree (%) 18.3 / 26.3

Caesium Binding Sites:

The binding sites of Caesium atom in the Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion (pdb code 2tpl). This binding sites where shown within 5.0 Angstroms radius around Caesium atom.
In total 2 binding sites of Caesium where determined in the Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion, PDB code: 2tpl:
Jump to Caesium binding site number: 1; 2;

Caesium binding site 1 out of 2 in 2tpl

Go back to Caesium Binding Sites List in 2tpl
Caesium binding site 1 out of 2 in the Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 1 of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cs500

b:2.0
occ:1.00
 O B:HOH666 2.9 25.5 1.0
O B:HOH636 2.9 17.7 1.0
O A:ASN262 2.9 12.1 1.0
O A:GLY52 3.0 8.8 1.0
OE1 B:GLU69 3.1 7.5 1.0
O A:HOH535 3.3 20.6 1.0
C A:GLY52 3.7 8.1 1.0
CA A:GLY52 3.9 8.3 1.0
C A:ASN262 3.9 11.9 1.0
CB A:ASN262 3.9 13.3 1.0
CB B:GLU69 4.0 8.3 1.0
O B:GLU69 4.0 10.1 1.0
CA A:ASN262 4.1 11.1 1.0
CD B:GLU69 4.2 8.0 1.0
CA B:ALA295 4.2 4.7 1.0
CA B:GLU69 4.4 10.6 1.0
CE A:LYS256 4.4 15.4 1.0
O B:HOH639 4.5 18.8 1.0
CG B:GLU69 4.6 4.6 1.0
CB B:ALA295 4.6 2.0 1.0
N B:GLY296 4.6 3.9 1.0
C B:GLU69 4.6 10.5 1.0
O B:LEU294 4.7 8.4 1.0
N A:THR53 4.7 6.9 1.0
O A:SER51 4.8 11.6 1.0
ND2 A:ASN262 4.8 13.5 1.0
CG A:ASN262 4.9 13.3 1.0
C B:ALA295 4.9 2.6 1.0

Caesium binding site 2 out of 2 in 2tpl

Go back to Caesium Binding Sites List in 2tpl
Caesium binding site 2 out of 2 in the Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 2 of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs500

b:2.0
occ:1.00
 O A:HOH571 2.9 31.2 1.0
O B:GLY52 3.0 8.6 1.0
O B:ASN262 3.1 12.7 1.0
O A:HOH515 3.1 13.3 1.0
OE1 A:GLU69 3.1 7.2 1.0
C B:GLY52 3.6 7.8 1.0
O A:GLU69 3.6 11.1 1.0
CB A:GLU69 3.8 9.3 1.0
CA B:GLY52 3.8 8.0 1.0
CB B:ASN262 4.0 9.0 1.0
C B:ASN262 4.0 12.3 1.0
CD A:GLU69 4.1 8.8 1.0
CA B:ASN262 4.2 10.9 1.0
NZ B:LYS256 4.2 24.9 1.0
CA A:GLU69 4.3 10.7 1.0
CA A:ALA295 4.3 5.2 1.0
O A:HOH534 4.3 20.4 1.0
CG A:GLU69 4.3 2.0 1.0
C A:GLU69 4.4 11.3 1.0
N B:THR53 4.6 7.6 1.0
N A:GLY296 4.6 3.8 1.0
CB A:ALA295 4.6 6.7 1.0
O B:SER51 4.7 12.4 1.0
ND2 B:ASN262 4.9 9.4 1.0
CG B:ASN262 4.9 9.5 1.0
CE B:LYS256 5.0 18.9 1.0
O A:LEU294 5.0 9.7 1.0

Reference:

B.Sundararaju, A.A.Antson, R.S.Phillips, T.V.Demidkina, M.V.Barbolina, P.Gollnick, G.G.Dodson, K.S.Wilson. The Crystal Structure of Citrobacter Freundii Tyrosine Phenol-Lyase Complexed with 3-(4'-Hydroxyphenyl)Propionic Acid, Together with Site-Directed Mutagenesis and Kinetic Analysis, Demonstrates That Arginine 381 Is Required For Substrate Specificity. Biochemistry V. 36 6502 1997.
ISSN: ISSN 0006-2960
PubMed: 9174368
DOI: 10.1021/BI962917+
Page generated: Tue Jul 30 20:20:32 2024

Last articles

Zn in 9JPJ
Zn in 9JP7
Zn in 9JPK
Zn in 9JPL
Zn in 9GN6
Zn in 9GN7
Zn in 9GKU
Zn in 9GKW
Zn in 9GKX
Zn in 9GL0
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy