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Caesium in PDB 2tpl: Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion

Enzymatic activity of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion

All present enzymatic activity of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion:
4.1.99.2;

Protein crystallography data

The structure of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion, PDB code: 2tpl was solved by A.A.Antson, T.V.Demidkina, K.S.Wilson, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 15.00 / 2.50
Space group P 21 21 2
Cell size a, b, c (Å), α, β, γ (°) 135.070, 143.910, 59.800, 90.00, 90.00, 90.00
R / Rfree (%) 18.3 / 26.3

Caesium Binding Sites:

The binding sites of Caesium atom in the Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion (pdb code 2tpl). This binding sites where shown within 5.0 Angstroms radius around Caesium atom.
In total 2 binding sites of Caesium where determined in the Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion, PDB code: 2tpl:
Jump to Caesium binding site number: 1; 2;

Caesium binding site 1 out of 2 in 2tpl

Go back to Caesium Binding Sites List in 2tpl
Caesium binding site 1 out of 2 in the Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 1 of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion within 5.0Å range:
probe atom residue distance (Å) B Occ
A:Cs500

b:2.0
occ:1.00
O B:HOH666 2.9 25.5 1.0
O B:HOH636 2.9 17.7 1.0
O A:ASN262 2.9 12.1 1.0
O A:GLY52 3.0 8.8 1.0
OE1 B:GLU69 3.1 7.5 1.0
O A:HOH535 3.3 20.6 1.0
C A:GLY52 3.7 8.1 1.0
CA A:GLY52 3.9 8.3 1.0
C A:ASN262 3.9 11.9 1.0
CB A:ASN262 3.9 13.3 1.0
CB B:GLU69 4.0 8.3 1.0
O B:GLU69 4.0 10.1 1.0
CA A:ASN262 4.1 11.1 1.0
CD B:GLU69 4.2 8.0 1.0
CA B:ALA295 4.2 4.7 1.0
CA B:GLU69 4.4 10.6 1.0
CE A:LYS256 4.4 15.4 1.0
O B:HOH639 4.5 18.8 1.0
CG B:GLU69 4.6 4.6 1.0
CB B:ALA295 4.6 2.0 1.0
N B:GLY296 4.6 3.9 1.0
C B:GLU69 4.6 10.5 1.0
O B:LEU294 4.7 8.4 1.0
N A:THR53 4.7 6.9 1.0
O A:SER51 4.8 11.6 1.0
ND2 A:ASN262 4.8 13.5 1.0
CG A:ASN262 4.9 13.3 1.0
C B:ALA295 4.9 2.6 1.0

Caesium binding site 2 out of 2 in 2tpl

Go back to Caesium Binding Sites List in 2tpl
Caesium binding site 2 out of 2 in the Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 2 of Tyrosine Phenol-Lyase From Citrobacter Intermedius Complex with 3-(4'- Hydroxyphenyl)Propionic Acid, Pyridoxal-5'-Phosphate and Cs+ Ion within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs500

b:2.0
occ:1.00
O A:HOH571 2.9 31.2 1.0
O B:GLY52 3.0 8.6 1.0
O B:ASN262 3.1 12.7 1.0
O A:HOH515 3.1 13.3 1.0
OE1 A:GLU69 3.1 7.2 1.0
C B:GLY52 3.6 7.8 1.0
O A:GLU69 3.6 11.1 1.0
CB A:GLU69 3.8 9.3 1.0
CA B:GLY52 3.8 8.0 1.0
CB B:ASN262 4.0 9.0 1.0
C B:ASN262 4.0 12.3 1.0
CD A:GLU69 4.1 8.8 1.0
CA B:ASN262 4.2 10.9 1.0
NZ B:LYS256 4.2 24.9 1.0
CA A:GLU69 4.3 10.7 1.0
CA A:ALA295 4.3 5.2 1.0
O A:HOH534 4.3 20.4 1.0
CG A:GLU69 4.3 2.0 1.0
C A:GLU69 4.4 11.3 1.0
N B:THR53 4.6 7.6 1.0
N A:GLY296 4.6 3.8 1.0
CB A:ALA295 4.6 6.7 1.0
O B:SER51 4.7 12.4 1.0
ND2 B:ASN262 4.9 9.4 1.0
CG B:ASN262 4.9 9.5 1.0
CE B:LYS256 5.0 18.9 1.0
O A:LEU294 5.0 9.7 1.0

Reference:

B.Sundararaju, A.A.Antson, R.S.Phillips, T.V.Demidkina, M.V.Barbolina, P.Gollnick, G.G.Dodson, K.S.Wilson. The Crystal Structure of Citrobacter Freundii Tyrosine Phenol-Lyase Complexed with 3-(4'-Hydroxyphenyl)Propionic Acid, Together with Site-Directed Mutagenesis and Kinetic Analysis, Demonstrates That Arginine 381 Is Required For Substrate Specificity. Biochemistry V. 36 6502 1997.
ISSN: ISSN 0006-2960
PubMed: 9174368
DOI: 10.1021/BI962917+
Page generated: Sun Dec 13 10:55:55 2020

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