Caesium in PDB 7k0b: The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step

Enzymatic activity of The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step

All present enzymatic activity of The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step:
4.2.1.20;

Protein crystallography data

The structure of The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step, PDB code: 7k0b was solved by E.Hilario, M.F.Dunn, L.J.Mueller, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 39.18 / 1.57
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 182.939, 58.379, 67.199, 90, 94.63, 90
R / Rfree (%) 19.9 / 23.6

Other elements in 7k0b:

The structure of The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step also contains other interesting chemical elements:

Chlorine (Cl) 7 atoms

Caesium Binding Sites:

The binding sites of Caesium atom in the The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step (pdb code 7k0b). This binding sites where shown within 5.0 Angstroms radius around Caesium atom.
In total 5 binding sites of Caesium where determined in the The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step, PDB code: 7k0b:
Jump to Caesium binding site number: 1; 2; 3; 4; 5;

Caesium binding site 1 out of 5 in 7k0b

Go back to Caesium Binding Sites List in 7k0b
Caesium binding site 1 out of 5 in the The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 1 of The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs419

b:20.8
occ:0.60
 CS B:CS419 0.0 20.8 0.6
CS B:CS419 1.2 36.5 0.4
O B:THR66 2.9 23.6 1.0
O B:THR71 3.0 19.7 1.0
O B:THR69 3.1 23.6 1.0
O B:HOH650 3.1 34.4 1.0
OG1 B:THR66 3.2 21.9 1.0
O B:HOH749 3.3 46.9 1.0
C B:THR66 3.7 23.4 1.0
CB B:THR66 3.8 22.1 1.0
O B:HOH840 3.9 30.6 1.0
C B:THR69 4.0 23.7 1.0
C B:THR71 4.1 19.2 1.0
OG1 B:THR69 4.2 20.9 1.0
N B:ALA67 4.4 23.3 1.0
CA B:THR66 4.4 22.6 1.0
N B:THR69 4.5 24.8 1.0
N B:THR71 4.5 19.5 1.0
CA B:ALA67 4.5 25.2 1.0
O B:HOH778 4.6 60.0 1.0
O B:HOH595 4.6 19.2 1.0
CA B:THR69 4.7 23.7 1.0
N B:GLY68 4.7 26.8 1.0
N B:ARG70 4.8 23.7 1.0
CA B:ARG70 4.9 23.7 1.0
C B:ARG70 4.9 22.0 1.0
CA B:THR71 5.0 19.1 1.0
N B:THR72 5.0 19.4 1.0
CG2 B:THR66 5.0 21.3 1.0

Caesium binding site 2 out of 5 in 7k0b

Go back to Caesium Binding Sites List in 7k0b
Caesium binding site 2 out of 5 in the The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 2 of The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs419

b:36.5
occ:0.40
 CS B:CS419 0.0 36.5 0.4
CS B:CS419 1.2 20.8 0.6
O B:THR71 3.3 19.7 1.0
O B:THR69 3.4 23.6 1.0
O B:HOH749 3.6 46.9 1.0
O B:HOH650 3.6 34.4 1.0
O B:HOH840 3.7 30.6 1.0
OG1 B:THR66 4.1 21.9 1.0
O B:THR66 4.1 23.6 1.0
C B:THR71 4.2 19.2 1.0
O B:HOH778 4.4 60.0 1.0
OG1 B:THR72 4.4 20.0 1.0
C B:THR69 4.4 23.7 1.0
N B:THR71 4.7 19.5 1.0
CB B:THR66 4.7 22.1 1.0
N B:THR72 4.8 19.4 1.0
CA B:ARG70 4.8 23.7 1.0
C B:ARG70 4.8 22.0 1.0
CA B:THR72 4.8 18.8 1.0
O B:HOH595 4.9 19.2 1.0
C B:THR66 5.0 23.4 1.0

Caesium binding site 3 out of 5 in 7k0b

Go back to Caesium Binding Sites List in 7k0b
Caesium binding site 3 out of 5 in the The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 3 of The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs420

b:22.2
occ:0.50
 CS B:CS420 0.0 22.2 0.5
CS B:CS420 1.0 23.1 0.3
CS B:CS420 2.8 46.1 0.2
OE1 B:GLU256 3.0 24.7 1.0
O B:GLY232 3.0 20.9 1.0
O B:GLY268 3.3 23.9 1.0
O B:SER308 3.3 22.7 1.0
O B:VAL231 3.4 19.3 1.0
C B:GLY232 3.9 19.9 1.0
CD B:GLU256 3.9 23.0 1.0
CG B:GLU256 4.1 21.9 1.0
CB B:VAL309 4.1 20.4 1.0
CG2 B:VAL309 4.1 20.8 1.0
CG1 B:VAL231 4.1 18.4 1.0
C B:VAL231 4.2 18.7 1.0
CA B:GLY232 4.2 19.4 1.0
C B:SER308 4.4 23.3 1.0
C B:GLY268 4.5 24.3 1.0
O B:PHE306 4.5 29.6 1.0
CB B:GLU256 4.6 21.0 1.0
CD1 B:LEU270 4.6 32.0 1.0
CD B:PRO257 4.6 20.2 1.0
N B:GLY232 4.6 19.1 1.0
CA B:VAL309 4.6 20.3 1.0
N B:LEU270 4.7 26.3 1.0
CB B:VAL231 4.9 18.4 1.0
CB B:LEU270 4.9 28.6 1.0
CG B:LEU270 5.0 31.4 1.0

Caesium binding site 4 out of 5 in 7k0b

Go back to Caesium Binding Sites List in 7k0b
Caesium binding site 4 out of 5 in the The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 4 of The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs420

b:23.1
occ:0.30
 CS B:CS420 0.0 23.1 0.3
CS B:CS420 1.0 22.2 0.5
CS B:CS420 1.9 46.1 0.2
O B:GLY268 2.9 23.9 1.0
O B:SER308 3.1 22.7 1.0
O B:GLY232 3.3 20.9 1.0
O B:PHE306 3.6 29.6 1.0
OE1 B:GLU256 3.6 24.7 1.0
O B:VAL231 3.9 19.3 1.0
CD1 B:LEU270 4.0 32.0 1.0
C B:GLY232 4.0 19.9 1.0
C B:GLY268 4.0 24.3 1.0
C B:SER308 4.3 23.3 1.0
CA B:GLY232 4.3 19.4 1.0
O B:LEU304 4.5 28.1 1.0
CD B:PRO257 4.5 20.2 1.0
CG B:LEU270 4.6 31.4 1.0
CD B:GLU256 4.6 23.0 1.0
CB B:VAL309 4.6 20.4 1.0
C B:VAL231 4.7 18.7 1.0
CA B:GLY268 4.7 24.9 1.0
C B:PHE306 4.7 30.8 1.0
N B:LEU270 4.8 26.3 1.0
CG B:GLU256 4.8 21.9 1.0
CB B:LEU270 4.8 28.6 1.0
N B:SER308 4.8 26.6 1.0
CD2 B:PHE306 4.9 32.0 1.0
CG2 B:VAL309 4.9 20.8 1.0
N B:GLY232 4.9 19.1 1.0
CG B:PRO257 4.9 20.0 1.0
CA B:VAL309 4.9 20.3 1.0
CB B:PHE306 5.0 31.4 1.0

Caesium binding site 5 out of 5 in 7k0b

Go back to Caesium Binding Sites List in 7k0b
Caesium binding site 5 out of 5 in the The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 5 of The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs420

b:46.1
occ:0.20
 CS B:CS420 0.0 46.1 0.2
CS B:CS420 1.9 23.1 0.3
O B:PHE306 2.7 29.6 1.0
CS B:CS420 2.8 22.2 0.5
O B:LEU304 2.8 28.1 1.0
O B:GLY268 3.2 23.9 1.0
N B:PHE306 3.5 33.2 1.0
C B:PHE306 3.7 30.8 1.0
C B:LEU304 3.9 28.3 1.0
C B:GLY268 4.0 24.3 1.0
CA B:GLY268 4.0 24.9 1.0
CG B:PRO257 4.0 20.0 1.0
CA B:PHE306 4.0 31.6 1.0
CB B:PHE306 4.1 31.4 1.0
O B:GLY232 4.1 20.9 1.0
O B:SER308 4.2 22.7 1.0
CA B:GLY232 4.2 19.4 1.0
CD B:PRO257 4.2 20.2 1.0
O B:VAL231 4.3 19.3 1.0
C B:GLY232 4.4 19.9 1.0
OG B:SER297 4.5 39.1 1.0
CD1 B:LEU270 4.6 32.0 1.0
C B:ASP305 4.6 35.9 1.0
CA B:ASP305 4.8 35.1 1.0
N B:ASP305 4.8 30.1 1.0
CA B:LEU304 4.8 25.9 1.0
CB B:SER297 4.8 36.9 1.0
N B:PRO307 4.9 30.9 1.0
CD2 B:PHE306 4.9 32.0 1.0

Reference:

E.Hilario, M.F.Dunn, L.J.Mueller. The Internal Aldimine Form of Salmonella Typhimurium Tryptophan Synthase Mutant Beta-Q114A with Cesium Ion at the Metal Coordination Site. A Random Beta-P270L Mutation Was Inserted During Pcr Step. To Be Published.
Page generated: Fri Sep 24 13:05:19 2021

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