Atomistry » Caesium » PDB 1av2-2j9x » 2j9x
Atomistry »
  Caesium »
    PDB 1av2-2j9x »
      2j9x »

Caesium in PDB 2j9x: Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A)

Enzymatic activity of Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A)

All present enzymatic activity of Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A):
4.2.1.20;

Protein crystallography data

The structure of Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A), PDB code: 2j9x was solved by H.Ngo, N.Kimmich, R.Harris, D.Niks, L.Blumenstein, V.Kulik, T.R.Barends, I.Schlichting, M.F.Dunn, with X-Ray Crystallography technique. A brief refinement statistics is given in the table below:

Resolution Low / High (Å) 19.63 / 1.9
Space group C 1 2 1
Cell size a, b, c (Å), α, β, γ (°) 183.212, 59.621, 67.389, 90.00, 94.63, 90.00
R / Rfree (%) 21.5 / 24

Caesium Binding Sites:

The binding sites of Caesium atom in the Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A) (pdb code 2j9x). This binding sites where shown within 5.0 Angstroms radius around Caesium atom.
In total 3 binding sites of Caesium where determined in the Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A), PDB code: 2j9x:
Jump to Caesium binding site number: 1; 2; 3;

Caesium binding site 1 out of 3 in 2j9x

Go back to Caesium Binding Sites List in 2j9x
Caesium binding site 1 out of 3 in the Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A)


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 1 of Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs1397

b:9.1
occ:0.50
 O B:GLY232 2.9 10.4 1.0
O B:GLY268 3.0 4.7 1.0
O B:PHE306 3.1 8.3 1.0
O B:SER308 3.2 5.2 1.0
O B:LEU304 3.5 4.6 1.0
O B:VAL231 3.6 4.8 1.0
C B:GLY232 3.6 8.2 1.0
CA B:GLY232 3.7 7.5 1.0
C B:GLY268 4.1 6.6 1.0
C B:PHE306 4.1 9.7 1.0
CD B:PRO270 4.2 9.4 1.0
CB B:PHE306 4.3 10.1 1.0
CD B:PRO257 4.4 2.3 1.0
C B:VAL231 4.4 6.1 1.0
N B:PHE306 4.4 8.2 1.0
C B:SER308 4.4 6.9 1.0
N B:GLY232 4.5 6.2 1.0
CA B:PHE306 4.5 9.4 1.0
C B:LEU304 4.5 6.6 1.0
CA B:GLY268 4.6 5.9 1.0
CG B:PRO257 4.6 3.2 1.0
CG B:PRO270 4.7 10.4 1.0
CD2 B:PHE306 4.8 14.1 1.0
OE2 B:GLU256 4.8 7.9 1.0
N B:GLY233 4.8 6.8 1.0
N B:SER308 4.9 8.2 1.0

Caesium binding site 2 out of 3 in 2j9x

Go back to Caesium Binding Sites List in 2j9x
Caesium binding site 2 out of 3 in the Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A)


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 2 of Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs1398

b:28.8
occ:1.00
 O B:GLY54 2.7 6.8 1.0
O B:PRO56 2.9 3.6 1.0
O B:HOH2055 3.5 18.6 1.0
C B:GLY54 3.8 6.6 1.0
C1 B:DMS1400 3.8 28.0 1.0
C B:PRO56 4.0 5.3 1.0
C B:ARG55 4.1 5.1 1.0
N B:PRO56 4.1 5.7 1.0
O B:HOH2056 4.2 9.7 1.0
O B:ARG55 4.2 4.9 1.0
CD B:PRO56 4.4 7.1 1.0
C2 B:DMS1400 4.5 27.5 1.0
CA B:GLY54 4.6 6.0 1.0
N B:ARG55 4.6 4.6 1.0
CA B:ARG55 4.6 5.9 1.0
CA B:PRO56 4.7 6.5 1.0
CG B:PRO56 4.8 7.4 1.0
O B:HOH2068 4.9 23.0 1.0
S B:DMS1400 4.9 29.0 1.0

Caesium binding site 3 out of 3 in 2j9x

Go back to Caesium Binding Sites List in 2j9x
Caesium binding site 3 out of 3 in the Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A)


Mono view


Stereo pair view

A full contact list of Caesium with other atoms in the Cs binding site number 3 of Tryptophan Synthase in Complex with Gp, Alpha-D,L-Glycerol- Phosphate, Cs, PH6.5 - Alpha Aminoacrylate Form - (Gp)E(A- A) within 5.0Å range:
probe atom residue distance (Å) B Occ
B:Cs1399

b:30.4
occ:0.70
 O B:THR66 3.1 11.6 1.0
O B:HOH2036 3.2 41.9 1.0
O B:THR71 3.3 7.7 1.0
O B:THR69 3.4 10.3 1.0
OG1 B:THR66 3.5 10.2 1.0
CB B:THR66 3.8 10.2 1.0
C B:THR66 3.8 12.1 1.0
OG1 B:THR69 4.1 6.1 1.0
C B:THR69 4.2 8.6 1.0
C B:THR71 4.2 6.8 1.0
N B:THR71 4.4 5.4 1.0
CA B:THR66 4.4 10.1 1.0
N B:ALA67 4.5 10.9 1.0
N B:THR69 4.6 12.1 1.0
CA B:ALA67 4.7 13.4 1.0
O B:HOH2057 4.8 4.2 1.0
C B:ARG70 4.9 6.7 1.0
CA B:THR69 4.9 9.7 1.0
N B:ARG70 4.9 5.8 1.0
CA B:THR71 4.9 6.3 1.0
CA B:ARG70 4.9 8.3 1.0
N B:GLY68 4.9 14.7 1.0
OG1 B:THR72 5.0 6.5 1.0

Reference:

H.Ngo, N.Kimmich, R.Harris, D.Niks, L.Blumenstein, V.Kulik, T.R.Barends, I.Schlichting, M.F.Dunn. Allosteric Regulation of Substrate Channeling in Tryptophan Synthase: Modulation of the L-Serine Reaction in Stage I of the Beta-Reaction By Alpha- Site Ligands. Biochemistry V. 46 7740 2007.
ISSN: ISSN 0006-2960
PubMed: 17559232
DOI: 10.1021/BI7003872
Page generated: Tue Jul 30 20:18:04 2024

Last articles

Zn in 9JYW
Zn in 9IR4
Zn in 9IR3
Zn in 9GMX
Zn in 9GMW
Zn in 9JEJ
Zn in 9ERF
Zn in 9ERE
Zn in 9EGV
Zn in 9EGW
© Copyright 2008-2020 by atomistry.com
Home   |    Site Map   |    Copyright   |    Contact us   |    Privacy